Isolation and characterization of human melanocytes, keratinocytes and fibroblasts from surgically discarded skin
2D cell culture and transfection methods
Development of 3D skin model (air-liquid interface culture)
Cell cytotoxicity (MTT assay) and proliferation (BrDU assay) assays
Cell signalling analysis by BRET/FRET and HTRF techniques
Nucleic acid extraction
Gene expression analysis by real-time PCR and droplet digital PCR
Protein analysis by Western blot
Protein interactions studies by imaging techniques (immunofluorescence and Proximity Ligation Assay)